trmD [2019-08-01 11:48:39]

tRNA methyltransferase, methylates G37 residue at the N1 position in a subset of tRNAs

Locus: BSU_16030

Isoelectric point: 5.31

Molecular weight: 27.56 kDa

Protein length: 243 aa

Gene length: 732 bp

Function: tRNA modification

Product: tRNA methyltransferase

Essential: yes

E.C.: 2.1.1.31

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
• Groups of genes → Essential genes

Gene

Coordinates: 1,675,171 1,675,902

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• guanosine³⁷ in tRNA + S-adenosyl-L-methionine --> H⁺ + N1-methylguanosine³⁷ in tRNA + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• RNA methyltransferase trmD family (single member, according to UniProt)

Structure

• 3KY7 (from Staphylococcus aureus, 62% identity)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ylxM-ffh-rpsP-ylqC-ylqD-rimM-trmD-rplS
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulation

  • the mRNA is processed between ffh and rpsP by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed